This compact, graduate-level text focuses on a key area of biophysical chemistry. Introduction to Macromolecular Binding Equilibria first describes the features of binding sites for small molecules and then presents a discussion of the formulation of binding isotherms for simple mathematical models. The book addresses special problems in describing isotherms for linear polymers and examines the complications of binding cooperativity and thermodynamic linkage interactions among multiple ligands. It highlights important aspects of experimental designs, key aspects of ligand binding to planar arrays of sites, and methods for model-free analysis data used to reveal the binding isotherm.