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Immunogenetics: Methods and Protocols

PUBLISHER Humana (05/28/2022)
PRODUCT TYPE Hardcover (Hardcover)

Description

1. The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease

Anton W. Langerak

2. Next-Generation Sequencing-Based Clonality Detection of Immunoglobulin Gene Rearrangements in B-Cell Lymphoma

Diede A.G. van Bladel, Jessica L.M. van der Last-Kempkes, Blanca Scheijen, Patricia J.T.A. Groenen on behalf of the EuroClonality Consortium

3. One-Step Next Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

Patrick Villarese, Chrystelle Abdo, Matthieu Bertrand, Florian Thonier, Mathieu Giraud, Mikal Salson, and Elizabeth Macintyre

4. Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

Vincent H.J. van der Velden, Lorenz Bastian, Monika Brggemann, Alina Hartmann, and Nikos Darzentas

5. Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR

Irene Della Starza, Cornelia Eckert, Daniela Drandi, and Giovanni Cazzaniga on behalf of the EuroMRD Consortium

6. Quality Control for IG/TR Marker Identification and MRD Analysis

Eva Fronkova, Michael Svaton, and Jan Trka

7. cfDNA-Based NGS IG Analysis in Lymphoma

Christiane Pott, Michaela Kotrova, Nikos Darzentas, Monika Brggemann, and Mouhamad Khouja on behalf of the EuroClonality-NGS Working Group

8. Targeted Locus Amplification as Marker Screening Approach to Detect Immunoglobulin (IG) Translocations in B-Cell Non-Hodgkin Lymphomas

Elisa Genuardi, Beatrice Alessandria, Aurora Maria Civita, and Simone Ferrero

9. Immunoglobulin/T-Cell Receptor Capture Strategy for Comprehensive Immunogenetics

James Peter Stewart, Jana Gazdova, Shambhavi Srivastava, Julia Revolta, Louise Harewood, Manisha Maurya, Nikos Darzentas, and David Gonzalez

10. Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia

Anne Langlois de Septenville, Myriam Boudjoghra, Clotilde Bravetti, Marine Armand, Mikal Salson, Mathieu Giraud, and Frederic Davi

11. NGS-Based B-Cell Receptor Repertoire Analysis in the Context of Inborn Errors of Immunity

Pauline A. van Schouwenburg, Mirjam van der Burg, and Hanna IJspeert

12. Generic Multiplex Digital PCR for Accurate Quantification of T-Cells in Copy Number Stable and Unstable DNA Samples

Rogier J. Nell, Willem H. Zoutman, Mieke Versluis, and Pieter A. van der Velden

13. Gene Engineering T-Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve, Mandy van Brakel, Rebecca Wijers, Reno Debets, and Dora Hammerl

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Product Format
Product Details
ISBN-13: 9781071621141
ISBN-10: 1071621149
Binding: Hardback or Cased Book (Sewn)
Content Language: English
More Product Details
Page Count: 607
Carton Quantity: 6
Product Dimensions: 7.00 x 1.31 x 10.00 inches
Weight: 2.84 pound(s)
Feature Codes: Illustrated
Country of Origin: NL
Subject Information
BISAC Categories
Medical | Immunology
Descriptions, Reviews, Etc.
jacket back
This open access book explores techniques for working in the field of immunogenetics, i.e. fundamental and translational research into the adaptive immune receptor repertoire. Many chapters are dedicated to lab protocols, bioinformatics, and immunoinformatics analysis of high-resolution immunome analysis, exemplified by numerous applications. Additionally, the newest technological variations on these protocols are discussed, including non-amplicon, single-cell, and cell-free strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Immunogenetics: Methods and Protocols covers a broad spectrum of methodologies for applications in research and clinical diagnostics to illustrate the impact that immunogenetics has achieved and will further expand in all fields of medicine, from infection and (auto)immunity, to vaccination, to lymphoid malignancy and tumor immunity.
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1. The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease

Anton W. Langerak

2. Next-Generation Sequencing-Based Clonality Detection of Immunoglobulin Gene Rearrangements in B-Cell Lymphoma

Diede A.G. van Bladel, Jessica L.M. van der Last-Kempkes, Blanca Scheijen, Patricia J.T.A. Groenen on behalf of the EuroClonality Consortium

3. One-Step Next Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

Patrick Villarese, Chrystelle Abdo, Matthieu Bertrand, Florian Thonier, Mathieu Giraud, Mikal Salson, and Elizabeth Macintyre

4. Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

Vincent H.J. van der Velden, Lorenz Bastian, Monika Brggemann, Alina Hartmann, and Nikos Darzentas

5. Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR

Irene Della Starza, Cornelia Eckert, Daniela Drandi, and Giovanni Cazzaniga on behalf of the EuroMRD Consortium

6. Quality Control for IG/TR Marker Identification and MRD Analysis

Eva Fronkova, Michael Svaton, and Jan Trka

7. cfDNA-Based NGS IG Analysis in Lymphoma

Christiane Pott, Michaela Kotrova, Nikos Darzentas, Monika Brggemann, and Mouhamad Khouja on behalf of the EuroClonality-NGS Working Group

8. Targeted Locus Amplification as Marker Screening Approach to Detect Immunoglobulin (IG) Translocations in B-Cell Non-Hodgkin Lymphomas

Elisa Genuardi, Beatrice Alessandria, Aurora Maria Civita, and Simone Ferrero

9. Immunoglobulin/T-Cell Receptor Capture Strategy for Comprehensive Immunogenetics

James Peter Stewart, Jana Gazdova, Shambhavi Srivastava, Julia Revolta, Louise Harewood, Manisha Maurya, Nikos Darzentas, and David Gonzalez

10. Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia

Anne Langlois de Septenville, Myriam Boudjoghra, Clotilde Bravetti, Marine Armand, Mikal Salson, Mathieu Giraud, and Frederic Davi

11. NGS-Based B-Cell Receptor Repertoire Analysis in the Context of Inborn Errors of Immunity

Pauline A. van Schouwenburg, Mirjam van der Burg, and Hanna IJspeert

12. Generic Multiplex Digital PCR for Accurate Quantification of T-Cells in Copy Number Stable and Unstable DNA Samples

Rogier J. Nell, Willem H. Zoutman, Mieke Versluis, and Pieter A. van der Velden

13. Gene Engineering T-Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve, Mandy van Brakel, Rebecca Wijers, Reno Debets, and Dora Hammerl

Show More
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Hardcover