This second volume is a collection of new and updated protocols to study miRNA functions in plants. Chapters guide readers from the identification and detection of plant miRNAs, bioinformatic analyses, to strategies for functional analysis of miRNAs and their targets. Furthermore, it contains a few introductory chapters on plant miRNA functioning and, on their conservation, and evolution. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Plant MicroRNAs: Methods and Protocols, Second Edition aims to provide protocols to help new researchers while also supporting established researchers to broaden the scope of their investigations.