This fully updated volume explores experimental methods to assess the complex interactions between Trypanosoma cruzi and its host and to study different aspects of T. cruzi and Chagas disease. The techniques presented, which touch on areas of bioinformatics, biochemistry, genetics, and cell biology research, have been recently developed or adapted to the T. cruzi system by leading experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, T. cruzi Infection: Methods and Protocols, Second Edition provides a comprehensive understanding of the basic biology of this parasite and its crosstalk with the host immune response that aims to inspire further understanding of Chagas disease epidemiology, diagnosis, treatment, and clinical evaluation.