This detailed volume brings together cutting-edge methods from proteomics, transcriptomics, bioinformatics, and functional genomics in order to capture the dynamic and multidisciplinary nature of the microprotein field. Divided into four sections, the book explores the detection and identification of microproteins; transcriptome-based discovery; computational, structural, and evolutionary insights; and functional and biomedical relevance. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microproteins: Methods and Protocols serves not only as a technical guide but also as an inspiration for further exploration into these once-hidden elements of the proteome.