This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.

Sarah L. Crittenden, Hannah S. Seidel, and Judith Kimble

2. Methods for Studying the Germline of the Human Parasite Schistosoma Mansoni

Julie N.R. Collins and James J. Collins III

3. Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells

Mayu Inaba and Yukiko M. Yamashita

4. Live Imaging of the Drosophila Testis Stem Cell Niche

Leah J. Greenspan and Erika L. Matunis

Dana Gancz and Lilach Gilboa

6. Live-Cell Imaging of the Adult Drosophila Ovary using Confocal Microscopy

Nevine A. Shalaby and Michael Buszczak

7. Measurement Of Mrna Poly(A) Tail Lengths In Drosophila Female Germ Cells and Germline Stem Cells

Aymeric Chartier, Willy Joly, and Martine Simonelig

8. Identification of Germline Stem Cells in Zebrafish

Bruce W. Draper

9. Primordial Germ Cell Isolation from Xenopus Laevis Embryos

Amanda M. Butler, Tristan Aguero, Karen M. Newman, and Mary Lou King

10. Single-Cell Lineage Analysis of Oogenesis in Mice

Lei Lei and Allan C. Spradling

11. Visualization and Lineage Tracing Of Pax7⁺ Spermatogonial Stem Cells in the Mouse

Gina M. Aloisio, Ileana Cuevas, Yuji Nakada, Christopher G. Pea, and Diego H. Castrillon

12. Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells

Aileen R. Helsel and Jon M. Oatley

13. Mouse Fetal Germ Cell Isolation and Culture Techniques

Cassy M. Spiller, Guillaume Burnet, and Josephine Bowles

14. Rattus Norvegicus Spermatogenesis Colony Forming Assays

F. Kent Hamra

15. Identification of Mouse Pirna Pathway Components Using Anti-MIWI2 Antibodies

Takamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi