Heterologous Gene Expression in E.Coli: Methods and Protocols
| PUBLISHER | Humana (05/04/2017) |
| PRODUCT TYPE | Hardcover (Hardcover) |
Part I: High-Throughput Cloning, Expression Screening, and Optimization
1. Recombinant Protein Expression in E. coli: A Historical Perspective
Opher Gileadi
2. N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools
Christopher D.O. Cooper and Brian D. Marsden
3. Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli
Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, and Tamar Unger
4. ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences
Philippe J. Mas and Darren J. Hart
5. Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters
Troy Taylor, John-Paul Denson, and Dominic Esposito
6. Optimization of Membrane Protein Production Using Titratable Strains of E. coli
Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, and Louise E. Bird
7. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions
Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikstrm, Joen Luirink, and Jan-Willem de Gier
8. High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B
Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, and Antti Vasala
Part II: Protein Purification and Solubility Enhancement
9. A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease
Hseyin Besir
10. A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli
Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, and Glenn F. King
11. Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter
Ekaitz Errasti-Murugarren, Arturo Rodrguez-Banqueri, and Jos Luis Vzquez-Ibar
12. Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality
Ario de Marco
13. Protein Folding Using a Vortex Fluidic Device
Joshua Britton, Joshua N. Smith, Colin L. Raston, and Gregory A. Weiss
14. Removal of Affinity Tags with TEV Protease
Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, and David S. Waugh
Part III:
Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
Part I: High-Throughput Cloning, Expression Screening, and Optimization
1. Recombinant Protein Expression in E. coli: A Historical Perspective
Opher Gileadi
2. N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools
Christopher D.O. Cooper and Brian D. Marsden
3. Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli
Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, and Tamar Unger
4. ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences
Philippe J. Mas and Darren J. Hart
5. Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters
Troy Taylor, John-Paul Denson, and Dominic Esposito
6. Optimization of Membrane Protein Production Using Titratable Strains of E. coli
Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, and Louise E. Bird
7. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions
Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikstrm, Joen Luirink, and Jan-Willem de Gier
8. High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B
Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, and Antti Vasala
Part II: Protein Purification and Solubility Enhancement
9. A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease
Hseyin Besir
10. A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli
Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, and Glenn F. King
11. Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter
Ekaitz Errasti-Murugarren, Arturo Rodrguez-Banqueri, and Jos Luis Vzquez-Ibar
12. Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality
Ario de Marco
13. Protein Folding Using a Vortex Fluidic Device
Joshua Britton, Joshua N. Smith, Colin L. Raston, and Gregory A. Weiss
14. Removal of Affinity Tags with TEV Protease
Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, and David S. Waugh
Part III: