This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of "seed stock," standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.

Preface...
Table of Contents...
Contributing Authors...

Part I Generic Themes in Stem Cell Banking

1. Stem Cell Banking: A Global ViewGlyn Stacey
2. Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell BankingTherse Kallur, Pontus Blomberg, Sonya Stenfelt, Kristian Tryggvason, and Outi Hovatta
3. Acquisition and Reception of Primary Tissues, Cells, or Other Biological SpecimensLyn E. Healy
4. Information ManagementAlberto Labarga, Izaskun Beloqui, and Angel G. Martin
5. Cryopreservation: Vitrification and Control Rate CoolingCharles J. Hunt
6. Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical ApplicationKevin Bruce, John D.M. Campbell, and Paul De Sousa
7. Ethics and Governance of Stem Cell BanksDon Chalmers, Peter Rathjen, Joy Rathjen, and Dianne Nicol
Part II Protocols for Pluripotent Stem Cell Banking
8. Derivation of Human Embryonic Stem CellsJeremy M. Crook, Lucy Kravets, Teija Peura, and Meri T. Firpo
9. Derivation of Human Induced Pluripotent Stem Cells in Chemically Defined MediumGuokai Chen and Mahendra Rao
10. Culture, Adaptation, and Expansion of Pluripotent Stem CellsJennifer L. Brehm and Tenneille E. Ludwig
11. Cryobanking Pluripotent Stem CellsJeremy M. Crook, Eva Tomaskovic-Crook, and Tenneille E. Ludwig
12. Genome Editing in Human Pluripotent Stem CellsJared Carlson-Stevermer and Krishanu Saha

Part III Protocols for Mesenchymal Stem Cell Banking
13. Isolation, Culture, and Expansion of Mesenchymal Stem CellsIzaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, and Angel G. Martin
14. Cryobanking Mesenchymal Stem CellsAndrs Pavn, Izaskun Beloqui, Juan M. Salcedo, and Angel G. Martin
Part IV Protocols for Human Neural Stem Cell Banking
15. Culturing and Cryobanking Human Neural Stem CellsJeremy M. Crook and Eva Tomaskovic-Crook