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Zymography: Methods and Protocols

PUBLISHER Humana (06/12/2017)
PRODUCT TYPE Hardcover (Hardcover)
Description
Part I: Introduction

1. Zymography Principles

Jeff Wilkesman and Liliana Kurz

Part II: Endopeptidase Zymography

2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

Hidetaro Yasumitsu

3. Cysteine Protease Zymography: Brief Review

Jeff Wilkesman

4. Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

Gavin Kernaghan and Michael Mayerhofer

5. Detection of Aspartic Proteinase Activities Using Gel Zymography

Handunge Kumudu Irani Perera

6. MMP Activity Detection in Zymograms

Pter Bencsik, Monika Bartekova, Anik Grbe, Krisztina Kiss, Jnos Plczi, Jana Radosinska, Gergő Szűcs, and Pter Ferdinandy

7. Characterization of Novel Collagenolytic Proteases

Goran Mucic, Brankica Raskovic, and Natalija Polovic

8. Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors

Zongli Ren, Juanjuan Chen, and Raouf A. Khalil

9. Detection and Characterization of Bacterial Proteinases Using Zymography

Madathiparambil G. Madanan and Ambili Mechoor

10. A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography

Shivcharan Prasad and Ipsita Roy

Part III: Reverse Zymography and In Situ Zymography

11. Reverse Zymography: Overview and Pitfalls

Kanika Sharma and Debasish Bhattacharyya

12. Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions

Aastha Chhabra and Vibha Rani

Part IV: 2D Zymography

13. Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography

Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, and Zezong Gu

14. Two-Dimensional Zymography of Proteases from Steatotic Duck Liver

Jeff Wilkesman, Mara Fernanda Padrn, Liliana Kurz, and Herv Rmignon

Part V: Special Zymography Cases

15. Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases after SDS-PAGE and Blotting

Silvia Ravera and Isabella Panfoli

16. Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel

Chang-Su Park, Dae-Ook Kang, and Nack
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Product Format
Product Details
ISBN-13: 9781493971091
ISBN-10: 1493971093
Binding: Hardback or Cased Book (Sewn)
Content Language: English
More Product Details
Page Count: 310
Carton Quantity: 11
Product Dimensions: 7.00 x 0.75 x 10.00 inches
Weight: 1.74 pound(s)
Feature Codes: Illustrated
Country of Origin: NL
Subject Information
BISAC Categories
Science | Life Sciences - Biochemistry
Dewey Decimal: 572
Descriptions, Reviews, Etc.
jacket back
This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.
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Part I: Introduction

1. Zymography Principles

Jeff Wilkesman and Liliana Kurz

Part II: Endopeptidase Zymography

2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

Hidetaro Yasumitsu

3. Cysteine Protease Zymography: Brief Review

Jeff Wilkesman

4. Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

Gavin Kernaghan and Michael Mayerhofer

5. Detection of Aspartic Proteinase Activities Using Gel Zymography

Handunge Kumudu Irani Perera

6. MMP Activity Detection in Zymograms

Pter Bencsik, Monika Bartekova, Anik Grbe, Krisztina Kiss, Jnos Plczi, Jana Radosinska, Gergő Szűcs, and Pter Ferdinandy

7. Characterization of Novel Collagenolytic Proteases

Goran Mucic, Brankica Raskovic, and Natalija Polovic

8. Zymography as a Research Tool in the Study of Matrix Metalloproteinase Inhibitors

Zongli Ren, Juanjuan Chen, and Raouf A. Khalil

9. Detection and Characterization of Bacterial Proteinases Using Zymography

Madathiparambil G. Madanan and Ambili Mechoor

10. A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography

Shivcharan Prasad and Ipsita Roy

Part III: Reverse Zymography and In Situ Zymography

11. Reverse Zymography: Overview and Pitfalls

Kanika Sharma and Debasish Bhattacharyya

12. Cell In Situ Zymography: Imaging Enzyme-Substrate Interactions

Aastha Chhabra and Vibha Rani

Part IV: 2D Zymography

13. Examination of Gelatinase Isoforms in Rodent Models of Acute Neurodegenerative Diseases Using Two-Dimensional Zymography

Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, and Zezong Gu

14. Two-Dimensional Zymography of Proteases from Steatotic Duck Liver

Jeff Wilkesman, Mara Fernanda Padrn, Liliana Kurz, and Herv Rmignon

Part V: Special Zymography Cases

15. Simultaneous Detection of Activity and Relative Molecular Mass of Adenylate Kinases after SDS-PAGE and Blotting

Silvia Ravera and Isabella Panfoli

16. Silver-Stained Fibrin Zymography: Separation of Proteases and Activity Detection Using a Single Substrate-Containing Gel

Chang-Su Park, Dae-Ook Kang, and Nack
Show More
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Hardcover