Subcellular Fractionation: A Laboratory Manual
| AUTHOR | Pryor, Paul; Pryor, Paul; Pryor, Paul et al. |
| PUBLISHER | Cold Spring Harbor Laboratory Press (11/30/2014) |
| PRODUCT TYPE | Paperback (Paperback) |
Description
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
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Product Format
Product Details
ISBN-13:
9781621820420
ISBN-10:
1621820424
Binding:
Paperback or Softback (Trade Paperback (Us))
Content Language:
English
More Product Details
Page Count:
260
Carton Quantity:
11
Product Dimensions:
8.50 x 0.60 x 10.90 inches
Weight:
1.70 pound(s)
Feature Codes:
Bibliography,
Index,
Maps,
Glossary,
Illustrated
Country of Origin:
US
Subject Information
BISAC Categories
Science | Life Sciences - Biochemistry
Science | Life Sciences - Cell Biology
Science | Microscopes & Microscopy
Dewey Decimal:
611.018
Library of Congress Control Number:
2014034658
Descriptions, Reviews, Etc.
publisher marketing
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
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