Mutagenesis is a vast field of study. The difficulty in comprehension of biochemical and molecular foundations of healthy life, and enthusiasm to find easy explanation demands advancement of skills like mutagenesis. The data in this book contains experiences of experts working in the field of mutagenesis. It explains appropriate investigational representations for testing spontaneous and induced mutations which are helpful for fundamental and translational study. It contains techniques in the direction of gene targeting, creating disease and pest opposing plants, generating temperature responsive molecular tools, comprehending mitochondrial mutagenesis, identifying anti-mutagens, and enhancing genetic insight into damaged immunity and disease. It even explains mutagenesis induced by DNA destruction. It also presents benefits of in-vitro transcription and transformation to yield proteins with point mutations, DNA-protein or protein-protein interaction. This book intends to provide some useful data to its readers so that they can understand mutagenesis better.