Justicia adhatoda is a natural plant with lot of medicinal properties. To evaluate the strength of antimicrobial effectiveness of the ethanolic extract of Justica adhatoda, Muller-Hinton agar was used as the base medium for screening of antibacterial activity and antibiotic sensitivity test. Four bacterial strains are used; Escherichia coli, Klebsiella species, Pseudomonas species, Staphylococcus aureus. Antibiotic sensitivity is based on disc diffusion method. The Pseudomonas species shows higher activity. It is a multi-drug resistance in human and animal pathogenic bacteria. This is less expensive, safe and an effective natural extract.