Since the pioneering contributions of scientists like Watson, Crick, and Sanger, all life sciences disciplines have witnessed a long journey from the small-scale manual to the large-scale, automated, high-throughput 'reading' nucleotide constituents of DNA. This book offers readers a new vision and overview of the international research on early day-to-present efforts on DNA sequencing, exemplifying its monumental impact on medicine, genetics, and biotechnology. We compiled chapters on an in-depth analysis of the manual to next-generation sequencing revolution with future innovative vision emphasizing the transformative potential of sequencing technologies helping personalized medicine and genetic editing. Hopefully, the readers will enjoy the development stages of DNA sequencing methods and tools, their applications, and their use with recent technological advancements from cutting-edge DNA sequencing and its future.