This book presents the proceedings of the NeuroIS Retreat 2025, May 26-28, Vienna, Austria, reporting on topics at the intersection of information systems (IS) research, neurophysiology, and the brain sciences. Readers discover the latest findings from top scholars in the field of NeuroIS, which offer detailed insights on the neurobiology underlying IS behavior, essential methods and tools, and their applications for IS, as well as the application of neuroscience and neurophysiological theories to advance IS theory.