Objective of this study was to determine the safety and quality parameters of transgenic corn (Ajeeb YG) compared with its near isogenic (Ajeeb). The PCR analysis showed the presence of 35S promoter and Cry1Ab gene sequences in the transgenic corn resulting in presence infectious CaMV35S which can cause disease, carcinogenesis, mutagenesis, reactivation of dormant viruses and even generation of new viruses. Also, the results revealed some differences in the compositional analysis and physicochemical properties between the isogenic and transgenic samples as some of them were positive and other negative. Moreover, existent of adverse histopathlogical changes in rats fed on transgenic corn compared with that fed on isogenic corn. Therefore, we recommend to not cultivate or import this corn variety and the subject deserves further investigations.