Irradiation is the significant way of sterilization of food from microbes. This study is conducted to determine the microbial load on organic chicken and their elimination by gamma radiation and enhance the shelf life of organic chicken and optimizing the radiation dose that can be set as a standard for the sterilization of organic chicken meat in Pakistan. The microbes which eliminated by radiation treatment are Campylobacter jejuni, Escherichia coli, Staphylococcus aureus, Salmonella spp. and Aeromonas hydrophila. In addition to this, acid and antibiotic resistant microbial load is also reduced by this treatment without causing any changes in nutritional, sensory and technical qualities of meat.