This book has been arranges in six part, one introduction, describes Actinomycetes, Taxonomy, Identification of actinomycetes. Part two, review of literature cover the view of different author and about the research have been done in actinomycetes. Third part, materials and method contain the standard protocol endoplasmic actinomycetes isolation, Physiological and Biochemical characterization, Biocontrol, Preservation and Molecular Characterization. Forth part, results in which all results show as fig. Fifth part, discussion and Six part, conclusion such as Endophytic actinomycetes isolated from medicinal plants show promising biocontrol potential and paly an important role in the medicinal properties of these plants. It will be useful for the researcher and students who work on Actinomycetes.