The book is a comprehensive guide designed for students, researchers, and laboratory professionals in the field of biochemistry. This book explores the core principles behind biochemical methods and offers detailed insights into modern experimental techniques such as spectroscopy, chromatography, electrophoresis, and molecular cloning. It emphasizes the practical applications of these tools in diagnostics, drug discovery, molecular biology, and metabolic research. By blending theory with hands-on guidance, the book serves as an essential resource for understanding and mastering the diverse instruments and methodologies that define contemporary biochemical investigation.