The purpose of this book was to investigate the factors affecting teachers in primary education to use YouTube in the teaching process.For the construction of the questionnaire and the interpretation of results used the theoretical variables of the Theory of predesigned Conduct (Theory of Planned Behavior) (Ajzen, 1991) and the Technology Acceptance Model (Technology Acceptance Model) (Davis et al., 1989) .The final sample was 219 primary school teachers who were working in all regions of Primary Education, Attica.The main conclusions of the investigation was that, teachers find helpful Youtube as tool in the educational process. There was also a positive effect on factors such as vitality, timeliness and adequacy, while most useful in the educational process they considered teachers greater age. Finally, the variable sex did not seem to have great difference in acceptance and use among men and women.The work results in recommendations for future research on the educational use of YouTube.