This book highlights the significance of nanosensors for applications in the biomedical and healthcare sector. The topics emphasize the fundamental principles of nanosensors, their fabrication process, and their applications in different areas, such as biosensing, point-of-care (POC) development, drug delivery, biomedical imaging, and tissue engineering. The chapters cover the advancements in these areas by considering personalized medicine, telemedicine, and artificial intelligence (AI)-driven biosensing strategies. The book not only focuses on the advancements, but also highlights the challenges in this area from both technical and regulatory perspectives. Its content is written by experienced subject experts in a manner suited to the general public and researchers who are interested in nanotechnology and its applications in biomedical.