Root biomodification using root conditioning agents represents a valuable strategy in contemporary dental practice, offering opportunities to improve treatment outcomes, enhance patient comfort, and promote tissue healing in various clinical scenarios. It is well established that the periodontally diseased root surface does not favour regeneration of the periodontium due to its surface characteristics. Demineralization has been shown to alter the diseased root surface, creating a more acceptable surface that can influence events in wound healing. Hence, root conditioning agents play a vital role in enhancing the outcomes of periodontal therapy by promoting tissue healing and regeneration.