Nanotechnology, a rapidly expanding field, showcases diverse applications across disciplines. Platinum nanoparticles (PtNPs) are a focal point in scientific exploration, offering non-toxicity and stability, especially in bioengineering. This concise review surveys global research on PtNPs, covering synthesis methods (Physical, Chemical, and Biological), along with in-vivo and in-vitro analyses. Nanometrological techniques for characterization are explored, aiding in understanding their physical and chemical attributes. The book extends to recent advancements in PtNP functionalization for biomedical purposes, including nanomedicine, combination therapy, cancer treatment, and antibacterial applications. The structured framework of the book comprises sections on Introduction, Platinum Nanoparticles, Synthesis, Characterization, Toxicology, Biomedical Industry, Antibacterial Applications, and a concluding perspective.