A comprehensive guide to LC-MS applications that probe the dynamic aspectS of proteomes

The systematic identification and characterization of proteins and proteomes is one of the major interdisciplinary research areas of the life sciences in the post-genomic era. One of the primary goals of current proteomic research is the description of the composition, dynamics, and connections of the multi-protein "modules" and "machineries" that perform a wide range of biological functions in the cell. Proteomic Biology Using LC-MS: Large Scale Analysis of Cellular Dynamics and Function examines the latest LC-MS technologies in depth and explores their application to the analysis of the dynamic aspects of cellular function. After an overview of proteomics and proteomic technologies, it covers:

• The basic aspects of LC-MS technology, including the principle of the method and assembly of the LC-MS system

• Integrated LC-based MS methodologies coupled with quantitative methodologies and/or bioinformatics

• The most recent applications, such as the analyses of signal transduction complexes following stimulation of cells and the analysis of the dynamic processes of ribosome biogenesis

• A new concept, the "Dynamome," or the expression of a comprehensive molecular set that participates in the whole dynamic process of a series of cellular events

Detailed experimental examples help readers explore various applications in action. With comprehensive coverage of LC-MS technologies applicable to the analysis of dynamic cellular functions in proteomic scale, this is a unique reference for investigators and laboratory staffs in academic institutions and industries. It is also a valuable resource for students as well as for scientists in other fields who want to bridge the gap between their specialties and proteomic biology.